Our lab designs and develops DNA-inspired Janus base nanomaterials (JBNs) for a variety of biomedical applications.
1) Janus bases nanotube (JBNt): molecular engineering using medicinal chemistry and computational modeling approaches.
Utilizing molecular design, organic synthesis and computational modeling approaches, we design, synthesize and characterize a family of small molecules with multiple hydrogen donor-acceptor pairs, named “Janus bases”. Mimicking DNA base pairs, these Janus bases can self-assemble into various 2D nanorosettes and 3D nanotubes (as shown below).
2) Janus base nano-coating (JBNc): electrically conductive and pizeoelectric coating for improved electrode surface and neurological applications.
For the first time, we developed an electrical conductive coating based on biomolecules—JBNc. The electrochemical properties of JBNc met or exceeded the standards of conventional gold and iridium oxide electrode surface. Moreover, JBNc can dramatically reduce the stiffness of the electrode surface to an extend close to the natural brain tissue. Especially, JBNc is a bioactive coating that can bioactivity promote neuron adhesion, neurogenesis, ion channel and anti-apoptosis.
3) Janus base nanopiece (JBNp): large mRNA delivery and Gene Editing.
Because JBNps are formed by non-covalent interactions of small molecule Janus base units, they present significantly lower cytotoxicity compared to conventional polymers and lipid nanoparticles (LNPs). More importantly, JBNps can escape from early endosomes via a pore formation mechanism. This is the molecular basis why JBNp can be more effective in large mRNA (such as Cas9 mRNA) delivery than conventional LNPs. With the ability of effective delivery of Cas9 mRNA, JBNp can be used as a powerful tool to achieve gene editing.
As below, the video below demonstrated green fluorescence labeled JBNp delivered into a cartilage cell.
4) Janus base nano-matrix (JBNm): tissue engineering and regenerative medicine on Earth and in space.
JBNm is an injectable scaffold formed by controlled assembly between JBNts, extracellular (ECM) proteins and growth factors. We can customize tissue-specific JBNms for different applications. For example, we have fabricated a cartilage-JBNm using matrilins (a cartilage ECM protein) and TGFβ (a cartilage growth factor). Importantly, JBNm is an injectable scaffold suitable for “difficult-to-reach” locations such as growth plate fractures in the center of a long bone and microchannels of microfluidic cartilage-on-a-chip.
Furthermore, we have pushed the boundary of bioengineering research bringing our technology from Earth into space. Supported by NASA, the International Space Station (ISS) national lab, NSF and other federal agencies, we develop and test our JBNs on the ISS via multiple space missions.
As below, the video demonstrated the self-assembly of JBNm in a biomimetic process without any chemical initiator or UV light.